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Indian Institute of Science Education and Research
Thiruvananthapuram

Prof Tapas K. Manna
Professor (Biology)
  +91 (0)471 - 2778172
  dG1hbm5hQGlpc2VydHZtLmFjLmlu

PUBLICATIONS

  1. Anand U, Bloomberg A, Bhattacharjee P, Mukhopadhyay S, Badarudeen B, Ramakrishnan S, Ben-David U, Manna TK.* FBXW7 E3 ligase prevents centriole overduplication by degrading the Plk4 phosphorylated STIL-SAS6 cartwheel assembly. (2026) J. Biol. Chem. 302, 111104.

  2. Mukhopadhyay S, Gao Q, Manna TK*, Schiebel E.* The gamma-TuRC: mastermind of multipurpose actions at the centrosome. (2026) Cell Mol. Life Sci. 83, 120 (review).

  3. Lakshmi RB, Nayak P, Raz L, Sarkar A, Saroha A, Kumari P, Nair VM, Kombarakkaran DP, Sajana S, M G S, Agasti SS, Paul R, Ben-David U, Manna TK.* (2024) CKAP-5 stabilizes CENP-E at kinetochore by regulating microtubule-chromosome attachment. EMBO Reports 25:1909-35. doi:10.1038/s44319-024-00106-9

  4. Nair VM, Sabu AS, Hussain A, Pauly DK, Lakshmi RB, Manna TK* (2023) E3-ubiquitin ligase, FBXW7 regulates mitotic progression by targeting BubR1 for ubiquitin-mediated degradation. Cell. Mol. Life Sci. 80, 374. doi: 10.1007/s00018-023-05019-9.

  5. Rajeev R, Mukhopadhyay S, Bhagyanath S, Devu Priya MRS, Manna TK.* (2023) TACC3-ch-TOG interaction regulates spindle microtubule assembly by controlling centrosomal recruitment of γ-TuRC. Biosci. Reports 43(3):BSR20221882. doi: 10.1042/BSR20221882

  6. Radhakrishnan RM, Kizhakkeduth ST, Nair VM, Ayyappan S, Lakshmi RB, Babu N, Prasannajith A, Umeda K, Vijayan V, Kodera N, Manna TK* (2022) Kinetochore-microtubule attachment in human cells is regulated by the interaction of a conserved motif of Ska1 with EB1. J. Biol. Chem., 299, 102853. 10.1016/j.jbc.2022.102853

  7. Badarudeen B, Anand U, Mukhopadhyay S, Manna TK* (2021) Ubiquitin signaling in the control of centriole duplication. FEBS J. 289:4830-4849. DOI: 10.1111/febs.16069.

  8. Ayyappan S, Dharan PS, Krishnan A, Marira RR, Lambert M, Manna TK, Vijayan V* (2021) SxIP binding disrupts the constitutive homodimer interface of EB1 and stabilizes EB1 monomer. Biophysical J., 120: 2019-2029. 10.1016/j.bpj.2021.03.004

  9. Gupta H, Rajeev R, Sasmal R, Radhakrishnan MR, Anand U, Agasti S, Manna TK* (2020) SAS-6 association with γ-tubulin ring complex is required for centriole duplication in human cells. Current Biology, 30: 2395-2403. 10.1016/j.cub.2020.04.036

  10. Badarudeen B, Gupta R, Nair SV, Chandrasekharan A, Manna TK * (2020) The ubiquitin ligase FBXW7 targets the centriolar assembly protein HsSAS-6 for degradation and thereby regulates centriole duplication. J. Biol. Chem., 295, 4428-4437. 10.1074/jbc.AC119.012178

  11. Rajeev R, Singh P, Asmita A, Anand U, Manna TK * (2019) Aurora a site specific TACC3 phosphorylation regulates astral microtubule assembly by stabilizing γ-tubulin ring complex. BMC Mol Cell Biol. 20: 58. 10.1186/s12860-019-0242-z

  12. Gireesh KK, Shine A, Bhagya Lakshmi R, Vijayan V*, Manna TK* (2018) GTP binding facilitates EB1 recruitment onto microtubules by relieving its auto-inhibition. Scientific Reports, 8: 9792. 10.1038/s41598-018-28056-y

  13. BhagyaLakshmi R, Nair VM, Manna TK*. (2018) Regulators of spindle microtubules and their mechanisms: living together matters. (review) IUBMB Life, 70, 101. 10.1002/iub.1708

  14. Thomas GE, Renjith MR, Manna TK*. (2017) Kinetochore-Microtubule Interactions in Chromosome Segregation: lessons from yeast and mammalian cells. Biochemical Journal, 474, 3559. 10.1042/BCJ20170518

  15. Thomas GE, Bandopadhyay K, Sutradhar S, MR Renjith, Singh P, KK Gireesh, Simon S, Badarudeen Binshad, Gupta H, Banerjee M, Paul R, Mitra J, Manna TK* (2016) EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice. Nature Communications, 7: 11665. 10.1038/ncomms11665

  16. Gupta H, Badarudeen Binshad, George A, Thomas GE, Manna TK.* (2015) Human SAS-6 C-terminus nucleates and promotes microtubule assembly in vitro by binding to microtubules. Biochemistry 54: 6413-22. 10.1021/acs.biochem.5b00978

  17. Suhail TV, Singh P, Manna TK.* (2015) Suppression of centrosome protein TACC3 induces G1 arrest and cell death through activation of p38-p53-p21 stress signaling pathway. Eur. J. Cell Biology, 94: 90-100. 10.1016/j.ejcb.2014.12.001

  18. Thomas GE, Sreeja JS, KK Gireesh, Gupta H, Manna TK.* (2015) Suppression of +TIP protein EB1 sensitizes cells to paclitaxel-induced proliferation inhibition and apoptosis through inhibition of paclitaxel binding on microtubules. Int. J. Oncol., 46, 133-146. 10.3892/ijo.2014.2701

  19. Singh P, Thomas GE, KK Gireesh, Manna TK.* (2014) TACC3 regulates microtubule nucleation by affecting gamma-tubulin ring complexes. J. Biol. Chem., 289, 31719-31735. 10.1074/jbc.M114.575100

  20. KK Gireesh, Sreeja JS, Chakraborti S, Singh P, Thomas GE, Gupta H, Manna TK.* (2014) Microtubule +TIP protein EB1 binds to GTP and undergoes dissociation from dimer to monomers on binding GTP. Biochemistry, 53, 5551-5557. 10.1021/bi5007942

  21. KK Gireesh, Rashid A, Chakraborty S, Panda D, Manna TK*. (2012) CIL-102 binds to tubulin at colchicine binding site and triggers apoptosis in MCF-7 cells by inducing monopolar and multinucleated cells. Biochemical Pharmacology, 84, 633-645. 10.1016/j.bcp.2012.06.008

  22. Manna T, Thrower DA, Honnappa S, Steinmetz MO, Wilson L. (2009) Regulation of microtubule dynamic instability in vitro by differentially phosphorylated stathmin. J. Biol. Chem., 284, 15640-15649. 10.1074/jbc.M900343200

  23. Sivaram M, Wadzinski, T, Redick S, Manna T, Doxsey, SJ (2009) Dynein light intermediate chain 1 is required for progress spindle assembly checkpoint. EMBO Journal, 28, 902-914. 10.1038/emboj.2009.38

  24. Manna T, Honnappa S, Steinmetz MO, Wilson L. (2008) Suppression of microtubule dynamic instability by the +TIP protein EB1 and its modulation by the CAP-Gly domain of p150glued. Biochemistry, 47, 779-786. 10.1021/bi701912g

  25. Ray A, Okouneva T, Manna T, Miller HP, Schmid SM, Art.haud L, Jordan MA, Wilson L. (2007) Mechanism of action of the microtubule targeted antimitotic depsipeptide Tasidotin (formerly ILX 651) and its major metabolite, Tasidotin C-carboxylate. Cancer Research, 67, 3767-3776. 10.1158/0008-5472.CAN-06-3065

  26. Manna T, Grenningloh G, Miller HP, Wilson L. (2007) The Oncoprotein18 family protein SCG10 differentially regulates plus and minus end dynamics of microtubule at steady state in vitro: Implications for its role in neurite outgrowth. Biochemistry, 46, 3543-3552. 10.1021/bi061819d

  27. Manna T, Thrower D, Miller HP, Curmi P, Wilson L. (2006) Oncoprotein18 strongly increases the minus end catastrophe frequency and induces rapid treadmilling of bovine brain microtubules at steady state in vitro. J. Biol. Chem., 281: 2071-2078. 10.1074/jbc.M510661200

  28. Mohan R, Banerjee M, Ray A, Manna T, Wilson L, Owa T, Bhattacharyya B, Panda D. (2006) Antimitotic sulfonamides inhibit microtubule assembly dynamics and cancer cell proliferation. Biochemistry, 45, 5440-5449. 10.1021/bi0523409

  29. Jordan MA, Kamath K, Manna T, Okouneva T, Miller HP, Davis C, Littlefield B, Wilson L. (2005) The primary antimitotic mechanism of action of the synthetic halichondrin E7389 is suppression of microtubule growth. Molecular Cancer Therapeutics, 4:1086-1095. 10.1158/1535-7163.MCT-04-0345

  30. Sarkar T, Mitra G, Gupta S, Manna T, Poddar A, Panda D, Das KP, Bhattacharyya B. (2004) MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes. Eur. J. Biochem. 271(8):1488-96. 10.1111/j.1432-1033.2004.04053.x.

  31. Bhattacharyya B, Manna T, Sarkar T, Poddar A. (2002) Chaperone-like activity of detergents, RNA and proteins Proc. Ind. Natl. Sci. Acad. (review) 68, 367-374.

  32. Manna T, Sarkar T, Poddar A, Roychowdhury M, Das KP, Bhattacharyya B. (2001) Chaperone-like activity of tubulin. binding and reactivation of unfolded substrate enzymes. J. Biol. Chem., 276, 39742-39747. 10.1074/jbc.M104061200

  33. Sarkar T, Manna T, Bhattacharyya S, Mahapatra P, Poddar A, Pena J, Solana R, Tarazona R, Roy S, Bhattacharyya B. (2001) Role of the carboxy-termini of tubulin on its chaperone-like activity. Proteins: Structure, Function, and Genetics 44, 262-269. 10.1002/prot.1091

  34. Pal D, Mahapatra P, Manna T, Chakraborty P, Bhattacharyya B, Banerjee A, Basu G, Roy S. (2001) Conformational properties of alpha-tubulin tail peptide: implications for tail-body interaction. Biochemistry 40, 15512-15519. 10.1021/bi015677t

  35. Roychowdhury M, Sarkar N, Manna T, Bhattacharyya S, Sarkar T, Basusarkar P, Roy S, Bhattacharyya B. (2000) Sulfhydryls of tubulin. A probe to detect conformational changes of tubulin. Eur. J. Biochem. 267, 3469-3476. 10.1046/j.1432-1327.2000.01369.x.

  36. Guha S, Manna T, Das, KP, Bhattacharyya B. (1998) Chaperone-like activity of tubulin. J. Biol. Chem., 273, 30077- 30080. 10.1074/jbc.273.46.30077.

  37. Bhattacharyya B, Manna T, Sarkar T, Poddar A. Chaperone-like activity of detergents, RNA and proteins (2002) Proc. Ind. Natl. Sci. Acad. (review) 68, 367-374.